This funding opportunity announcement (FOA), entitled “Productivity Enhanced Algae and Tool-Kits,” has two topic areas: (1) algal strain improvements and (2) algal cultivation biology improvements. This FOA will allow the selection of a variety of projects and approaches that overcome species-specific, ecological, and practical challenges to improved algal productivity and biomass composition—two key metrics in achieving high fuel yields. Successful projects will advance to a cultivation readiness challenge by the end of their development efforts to demonstrate their peak productivity potential in an outdoor field trial. The objective of this FOA is the development of productivity-enhancing algal biology technologies that can enable doubling the current state of technology for seasonal algal biomass productivities. Successful projects will also conduct limited techno-economic and life-cycle analyses of their proposed approaches to help understand commercialization pathways for their technologies.